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On the basis of the fact which Dimethyl sulfoxide(DMSO) penetrate tissue rapidly and reduce the size of ice crystals, a test was done to know whether DMSO can be used in the process of frozen sections. Small blocks of liver, Kidney, lung and spleen tissues were dissected form rats and were divided each into four portions. One block of each tissue was placed for 20 to 25 minutes in each of the following solutions : normal saline(NS), 5%(V/V) DMSO in NS, 10% DMSO in NS, and 20% DMSO in NS. Frozen sections were done on these tissues, and then hematoxylin-eosin stain was done and compared. Generally there was less tissue disruption in the samples treated with DMSO than without DMSO. Especially, in all the tissues, the 10% DMSO gave the best results in staining, cutting and the thickness of the tissue sections. Tissue treated with 20% DMSO was very hard to cut and could not be cut as thinly as the 5% or 10% DMSO treated tissue. Especially in the liver tissue, shrinkage of tissue sections was occured. The kidney tissues treated with 5% DMSO and 10% DMSO were comparable in reducing tissue disruption. The DMSO does not interfere with formalin fixation after frozen sections in the process of routine preparation. The time of penetration of the DMSO into the tissue block was very fast and penetration was apparently completed by ten minutes. If the time of treatment with DMSO was longer, it stained darkerly with hematoxylin-eosin. All of these findings suggest that treatment with 10%(V/V) DMSO for frozen sections enhances the quality of the frozen sections. If possible, this method should be used for human pathologic specimens through the examination on applying DMSO to human tissues.
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